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Correction of Hair Shaft Defects through Allele-Specific Silencing of Mutant Krt75

2017年12月17日 来源:

Liu YSnedecor ERZhang XXu YHuang LJones ECZhang LClark RARoop DRQin CChen J.

Abstract

       Dominant mutations in keratin genes can cause a number of inheritable skin disorders characterized by intraepidermal blistering, epidermal hyperkeratosis, or abnormalities in skin appendages, such as nail plate dystrophy and structural defects in hair. Allele-specific silencing of mutant keratins through RNA interference is a promising therapeutic approach for suppressing the expression ofmutant keratins and related phenotypes in the epidermis. However, its effectiveness on skin appendages remains to be confirmed in vivo. In this study, we developed allele-specific small interfering RNAs capable of selectively suppressing the expression of a mutantKrt75, which causes hair shaft structural defects characterized by the development of blebs along the hair shaft in mice. Hairregenerated from epidermal keratinocyte progenitor cells isolated from mutant Krt75 mouse models reproduced the blebbing phenotype when grafted in vivo. In contrast, mutant cells manipulated with a lentiviral vector expressing mutant Krt75-specific short hairpin RNA (shRNA) persistently suppressed this phenotype. The phenotypic correction was associated with a significant reduction ofmutant Krt75 mRNA in the skin grafts. Thus, data obtained from this study demonstrated the feasibility of utilizing RNA interference to achieve durable correction of hair structural phenotypes through allele-specific silencing of mutant keratin genes.

全文下载:http://www.sciencedirect.com/science/article/pii/S0022202X15000251?via%3Dihub