GC00011

Accession No

GC00011

Strain Name

SD.Prf1(tm)-GC/ILAS

Status

Freezed embryo

Gene/full name

Prf1 / perforin 1

Gene ID

50669

Background

Sprague Dawley

Type

Knockout

Donor

Lianfeng Zhang, Yuanwu Ma, Xu Zhang, Wei Chen

Disease & Phenotype

immune system diseases

Construction Description

Genotyping Protocols

PRF1-F  TGCTACACTGCCACTCGGTCAG

PRF1-R  CTAGACCACTTAGAGTGTCCCAC

扩增片断535bp,敲除片断较小,需测序

Mating System

Heterozygote x Heterozygote;

SPF (specific pathogen-free) conditions are recommended.

Reference

1. Yuanwu Ma,Bin Shen,Xu Zhang. Heritable Multiplex Genetic Engineering in Rats Using CRISPR/Cas9, PLoS One. 2014; 9(3): e89413.