GC00209

GC00209

SD. MT-Trn E(G14098A)/MT-Trn K(G7755A)-GC-ILAS

Accession No

GC00209

Strain Name

SD. MT-Trn E(G14098A)/MT-Trn K(G7755A)-GC-ILAS

Status

Frozen Embryo

Gene/full name  

MT-Trn E; MT-Trn K

Gene ID  

170619; 170615

Background

Sprague Dawley

Type

Mutation

Donor

Yuanwu Ma, Xiaolong Qi, Xu Zhang, Wei Chen， Lianfeng Zhang

Disease & Phenotype  

Mitochondrial Disease

Construction Description

Genotyping Protocols

突变检测引物：

7755-F：TGCTCTGAAATTTGCGGCTC

7755-R：AGTCGGTTGCTGATTAGGCG

14098-F：TCAAGTCTCCGGGTACTCCT

14098-R：AAATATTGAGGCGCCGTTGG

Standard PCR

PCR条件：94℃ 5min;(94℃ 20s, 60℃ 30s, 72℃ 1min) 30 cycle; 72℃ 10min; 4℃ ∞

Mating System

Mutant rat（♀）cross with wildtype SD rat（♂）;

Maternal inherited

Reference