miR-29c regulates BACE1 protein expression.

Brain Res. 2011 Jun 13;1395:108-15. doi: 10.1016/j.brainres.2011.04.035. Epub 2011 Apr 22.

      miR-29c regulates BACE1 protein expression.

      Zong Y, Wang H, Dong W, Quan X, Zhu H, Xu Y, Huang L, Ma C, Qin C.

      Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center, Peking Union Medical Collage (PUMC), No. 5, Panjiayuan, Nanli, Chaoyang District, Beijing 10021, PR China.

Abstract

      Recently, BACE1 expression was shown to be regulated by microRNAs, small endogenous RNA molecules that regulate protein expression through sequence-specific interaction with messenger RNA. Here, we showed that microRNA-29c (miR-29c), a miRNA that is enriched in the brain and highly expressed in the APPswe/PSΔE9 mouse lowers BACE1 protein in vitro and in transgenic miR-29c mice. In silico analysis identified two putative target sites in the BACE1 mRNA for the miR-29c family. We chose SH-SY5Y, HEK-293T cell lines and miR-29c transgenic mice for these studies to validate our hypothesis. Significantly, over-expression of miR-29c in SH-SY5Y, HEK-293T cell lines and miR-29c transgenic mice downregulated BACE1 protein levels. Our findings suggest that miR-29c may be an endogenous regulator of BACE1 protein expression.

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