AIDS Research Group

Overview:

Through 30 years of efforts, the AIDS research group has systematically established a standardized animal model system for HIV/AIDS in rhesus monkeys in China, especially by chairing a number of special projects and sub-projects on major infectious diseases in the 11th, 12th and 13th five-year Plans. For AIDS animal model research, the  research group focused on aspects as followings, HIV/AIDS model closely related to virus strain, routes of infection, modes of  infection, susceptible animals, testing standard and evaluation system, etc., to integrate innovative research, include: To select, prepare and identify a large number of standardized monkey and human/monkey (chimeric) immunodeficiency viruses, analyze and compare different infection routes, innovate infection modes, screen non-human primates with clear genetic background, improve model standards and standardize model analysis system. On this basis, extensive model application will be carried out to form a comprehensive HIV/AIDS primate model research system covering technology, talents and application.
 

46 SHIV strains and pseudovirus plasmids derived from SIV and different subtypes of ENV and 18 HIV-sensitive cells were stored. 15 animal models of AIDS with different strains and different infection routes were systematically and normatively established. The virus used in these models covers the SIV and the source of different subtypes env SHIV strain, which use a different ways of infection, including vein (simulate drug taking), rectum (simulated homosexual behavior) and vagina (simulate heterosexual sex). This research group has published dozens of papers in Immunity, PLoS Pathog, J Virol, AIDS and other classic journals in this field, and has evaluated some of AIDS vaccines and drugs in China, which is a key platform for the in-vivo evaluation of AIDS vaccines and drugs in China. The establishment and application of primate model of AIDS research system won the third prize of the Beijing municipal science and technology prize, and the first prize of science and technology of Chinese Association for Laboratory Animal Sciences, They have made outstanding contributions for basic and applied research on HIV/AIDS in China.

 Research fields：

Research on the pathogenic mechanism and anti-infection immunity of AIDS.

1) Research on the creation, application and pathogenic mechanism of AIDS animal model;

2) Studies on immune regulation mechanism, apoptosis mechanism and signal pathway of pathogen infected host cells;

3) The interaction between host immunity and HIV/SIV and its relationship with disease progression and clinical outcome.

Research group members:

One professor, one associate professor, one associate chief technologist, and three other technical personnel

Wei Qiang PI

Researcher, doctoral supervisor

He is mainly engaged in the construction of primate models of AIDS and the pathogenesis and evaluation of vaccine of AIDS. SIV and SHIV virus animal models of Chinese rhesus monkeys were successfully established to determine the experimental indicators for drug screening and vaccine evaluation. Animal models of human major emerging infectious diseases and experimental comparative studies as well as experimental indicators for drug screening and vaccine evaluation were established.

 

Xue Jing associate professor		Cong Zhe
Chen Ting	Lu Qiuhan	Lu Jiahan
Li Guocui postgraduate	Luo Jiahui postgraduate	Tian Long postgraduate
Tong Ling postgraduate	Zhang Jingjing postgraduate	Zhang Lili postgraduate

Tel:010-67776049

 

Main research achievements:

 

　　1)Chong H(#), Xue J(#), Zhu Y(#), Cong Z, Chen T, Wei Q, Qin C*, He Y*. Monotherapy with a low-dose lipopeptide HIV fusion inhibitor maintains long-term viral suppression in rhesus macaques. PLoS Pathog. 2019 Feb 4;15(2):e1007552.

　　2)Chong H(#), Xue J(#), Zhu Y, Cong Z, Chen T, Guo Y, Wei Q, Zhou Y, Qin C*, He Y*. Design of Novel HIV-1/2 Fusion Inhibitors with High Therapeutic Efficacy in Rhesus Monkey Models. J Virol. 2018 Jul 31;92(16).

　　3)Li KL, Cong Z, Peng ZY, Chen T, Xue J*, Wei Q*. CD45RO regulates the HIV-1 gp120-mediated apoptosis of T cells by activating Lck. Biol Chem. 2018 May 24;399(6):583-591.

　　4)Chong H(#), Xue J(#), Xiong S, Cong Z, Ding X, Zhu Y, Liu Z, Chen T, Feng Y, He L, Guo Y, Wei Q, Zhou Y, Qin C*, He Y*. A Lipopeptide HIV-1/2 Fusion Inhibitor with Highly Potent In Vitro, Ex Vivo, and In Vivo Antiviral Activity. J Virol. 2017 May 12;91(11).

　　5)He H(#), Xue J(#), Wang W, Liu L, Ye C, Cong Z, Kimata J, Qin C*, Zhou P*. Efficient Transduction of Human and Rhesus Macaque Primary T Cells by a Modified Human Immunodeficiency Virus Type 1-Based Lentiviral Vector. Hum Gene Ther. 2017 Mar;28(3):271-285.

　　6)Xue J, Fu C, Cong Z, Peng L, Peng Z, Chen T, Wang W, Jiang H, Wei Q*, Qin C*. Galectin-3 promotes caspase-independent cell death of HIV-1-infected macrophages. FEBS J. 2017 Jan;284(1):97-113.

　　7)Bomsel M1, Tudor D, Drillet AS, Alfsen A, Ganor Y, Roger MG, Mouz N, Amacker M, Chalifour A, Diomede L, Devillier G, Cong Z, Wei Q, Gao H, Qin C, Yang GB, Zurbriggen R, Lopalco L, Fleury S. Immunization with HIV-1 gp41 subunit virosomes induces mucosal antibodies protecting nonhuman primates against vaginal SHIV challenges. Immunity. 2011 Feb 25;34(2):269-80.

　　8)Ren Y1, Li L, Wan Y, Wang W, Wang J, Chen J, Wei Q, Qin C, Xu J, Zhang X. Mucosal Topical Microbicide Candidates Exert Influence on the Subsequent SIV Infection and Survival by Regulating SIV-Specific T-Cell Immune Responses. J Acquir Immune Defic Syndr. 2016 Feb 1;71(2):121-9.

　　9)Liu L, Wei Q, Alvarez X, Wang H, Du Y, Zhu H, Jiang H, Zhou J, Lam P, Zhang L, Lackner A, Qin C, Chen Z. Epithelial cells lining salivary gland ducts are early target cells of severe acute respiratory syndrome coronavirus infection in the upper respiratory tracts of rhesus macaques. J Virology. Apr. 2011, p. 4025–4030.

　　10)Xue J, Cong Z, Xiong J, Wang W, Jiang H, Chen T, Wu F, Liu K, Su A, Ju B, Chen Z, Couto MA, Wei Q, Qin C. Repressive effect of primary virus replication on superinfection correlated with gut-derived central memory CD4(+) T cells in SHIV-infected Chinese rhesus macaques. PLoS One. 2013 Sep 2;8(9):e72295.

　　11)Xue J, Gao XQ, Fu CY, Cong Z, Jiang H, Wang W, Chen T, Wei Q, Qin C. Regulation of galectin-3-induced apoptosis of Jurkat cells by both O-glycans and N-glycans on CD45. FEBS Lett. 2013 Dec 11;587(24):3986-94.

　　12)Xue J, Zhu LP, Wei Q. IgG-Fc N-glycosylation at Asn297 and IgA O-glycosylation in the hinge region in health and disease. Glycoconj J. 2013 Nov;30(8):735-45.