Microecological Transformation Research group

Overview:

Using sterile animals as the carrier, the research group studied the relationship among neurodegenerative diseases, cardiovascular diseases and intestinal flora, as well as the relationship between intestinal fungi and diseases. In collaboration with other internal and external research groups, the influence of intestinal flora on peripheral lymphatic organs has been published. This study confirmed the correlation between intestinal flora and the development of peripheral lymphatic organs. RALDH+ DC cells in the intestinal tract migrated to peripheral lymph nodes after colonization, and regulated the structure and function of peripheral lymph nodes through retinoic acid signaling pathway. Currently, they are carrying out or have completed a number of major projects of the Ministry of Science and Technology, university-research cooperation projects of co-construction plan by Beijing Education Commission, Central Public-interest Scientific Institution Basal Research Fund, major collaborative innovation projects of the Chinese Academy of Medical Sciences, and national and provincial scientific research projects of National Natural Science Foundation of China. It provides sterile animals and experimental platforms for many research institutes all over the country. The results provide an animal model for the development and evaluation of hand-foot-and-mouth vaccine, rotavirus vaccine and human hepatitis E vaccine. The formation of socially shared sterile animal and pathogenic animal resources and comparative medical technology resources provides a platform for the study of the relationship between intestinal microbes and diseases, and provides a strong technical support for the medical research in China.

The Research Direction:

Using a series of animal models of human flora and an animal model of non-bacterial human diseases as tools, the comparative medical research on intestinal microbes, metabolism and immunity was carried out to expound the relationship between the structural changes of intestinal flora and the pathogenesis of human diseases. Research contents and available technical services include: 1. Establish an animal model similar to the phenotype of human diseases through the use of sterile technology and bacterial colony transplantation; 2. Animal models and aseptic techniques were used to provide platform support for the research on the relationship between intestinal flora and diseases, and the efficacy evaluation of microecological preparations.

Staff Composition:

Zhu Hua (PI)	Su Lei	Du Xiaopeng	Guo Yaqian

Scientific Research Achievements

 

Main papers published:

　　1、Peripheral Lymphoid Volume Expansion and Maintenance Are Controlled by Gut Microbiota via RALDH+ Dendritic Cells, Immunity. 2016 Feb 16;44(2):330-42. doi: 10.1016/j.immuni.2016.01.004

　　2、Interaction betweengut microbiome and mucosal immunity[J]. Military Medical Research. 2017, 27: 4-14.

　　3、Applications of next-generation sequencing in systemic autoimmune diseases[J]. Genomics Proteomics Bioinformatics. 2015 13: 242–249.

　　4、 The function of hematopoietic stem cells is altered by both genetic and inflammatory factors in lupus mice[J]. Blood. 2013, 121(11): 1986-1994.

　　5、 Rupestriomyces and Spissiomyces, two new genera of rock-inhabiting fungi from China[J]. Mycologia. 2015, 107(4): 831-844.

　　6、Phialemoniopsis endophytica sp. nov., a new species of endophytic fungi from Luffa cylindrica in Henan, China[J]. Mycol Prog. 2016, 15(5): 1-7.

　　7、Phylogenetic analysis of Plectosphaerella species based on multi-locus DNA sequences and description of P. sinensis sp. nov[J]. Mycol Prog. 2017, 16(8): 823-829.

　　8、The microbial community dynamics during the vitex honey ripening process in the honeycomb[J]. Front Microbiol. 2017, 8: 1649.

　　9、Multilocus phylogenetic analysis of Talaromyces species isolated from cucurbit plants in China and description of two new species, T. cucurbitiradicus and T. endophyticus [J]. Mycologia. 2018, 110(2): 375-386.

　　10、The Intestinal Microbiome and Alzheimer’s Disease：A Review[J].Animal Model

Exp Med,2018;1:180-188.

　　11、Lecanicillium coprophilum (Cordycipitaceae, Hypocreales), a new species from

the feces of Marmota monax in China. Phytotaxa[J].https://doi.org/10.11646/

phytotaxa.00.0.0

　　12、STZ诱导糖尿病大鼠模型肠道菌群变化.中国实验动物学报[J]. 2018(3): 229-233.

　　13、基于高通量测序的不同年龄恒河猴肠道菌群结构差异分析.中国实验动物学报[J].2019(1):79-85..

　　专利：

　　1、无菌大鼠的繁育饲料配方，200510086582.0，中国发明专利

　　2、无菌豚鼠的培育技术及饲料配方200510086583.5，中国发明专利

　　3、无菌隔离器 200520114540.9，中国实用新型专利

　　4、无菌兔的饲料配方及灭菌方法 200510086581.6中国发明专利

　　5、一种无菌大小鼠人工乳及其制备方法；201810283934.9，中国发明专利

　　6、一种不同日龄无菌大小鼠人工哺乳针头及其制备方法201810523297.8，中国发明专利

　　论著：

　　1、悉生动物学 王荫槐主编 辽宁大学出版社 2007年12月出版

　　2、悉生动物技术，实验动物人才培训教材

　　3、悉生蚕的培育及其在实验动物科学中的应用    王荫槐主编 天津出版传媒集团 2013年出版

　　获得奖励：

　　1、YDG型隔离器的研制，中国医学科学院中国协和医科大学科技成果奖

　　2、无菌兔、豚鼠的培养及研究：（1）无菌兔的培育和研究，中华人民共和国卫生部科学技术进步二等奖

　　3、无菌兔、豚鼠的培养及研究：（2）无菌豚鼠的饲养方法及其特征和应用的研究，中华人民共和国卫生部科学技术进步二等奖

　　4、无菌兔的培育和研究 中华人民共和国国家科学技术委员会，国家科技成果

　　5、无菌豚鼠的饲育方法及其特征和应用的研究 国家科学技术委员会科技成果

　　6、无菌大鼠的开发与应用研究 卫生部科学技术进步三等奖